Remus Th. Dame | Molecular Genetics

Universiteit Leiden

Remus Th. Dame, Ph.D.

Position:

Assistant professor

Research area:

Chromatin organisation & dynamics

Research theme:

Chromatin Structure & Epigenetics

Telephone number: +31 (0)71 527 5605
E-mail address: rtdame@chem.leidenuniv.nl
Faculty/Unit: Faculty of Science, Leiden Institute of Chemistry, Molecular Genetics
Office address: Gorlaeus Laboratories
Einsteinweg 55
2333 CC Leiden
roomnumber 0.3.31

Biography

Education

1997: MSc, Biochemistry, University of Amsterdam, The Netherlands.
2003: PhD, Molecular genetics, Leiden University, The Netherlands.

Positions held

2003 - 2006: Post-doc, Single-molecule biophysics, VU University, Amsterdam, The Netherlands.
2006 - 2009: Research Fellow, Single-molecule biophysics, VU University, Amsterdam, The Netherlands.
2007: Visiting Scientist, Cornell University, USA
2009 - : Assistant Professor, Lab of Molecular Genetics, Leiden University, The Netherlands

Awards

2005: VENI award (Innovational Research Incentive Scheme, NWO).
2008: VIDI award (Innovational Research Incentive Scheme, NWO).

Current Funding

- FES nano/biotechnology programme HTS&M (NL)
- NWO, Earth and Life Sciences, Innovational Research Incentive Scheme (NL)
- Royal Society International Joint Project Grant (UK)

2012

Westra, E.R., van Erp, P.B.G., Wong, S.P., Künne, T., Seegers, C.., Bollen, S.J., Jore, M.M., Semenova, E., Severinov, K., de Vos, W.M., Dame, R.T., de Vries, R., Brouns, S.J.J., van der Oost, J. , CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3, Mol Cell. In press., no. 1.

Liu, Z.F., Galli, F., Waterreus, W.J., Meulenbroek, E., Zhou, X., Lamers, G., Olsthoorn, R.C., Pannu, N.S., Oosterkamp, T., Dame, R.T., Abrahams, J.P. , Single walled carbon nanotubes as a scaffold to obtain physiological DNA concentrations., ChemPhysChem In press., no. 1.

2011

Sznee, K., Dekker, J.P., Dame, R.T., van Roon, H., Wuite, G.J., Frese, R.N. , Jumping mode atomic force microscopy on grana membranes from spinach, The Journal of biological chemistry, vol. 286, no. 45, pp. 39164-71.

Dame, R.T., Kalmykowa, O.J., Grainger, D.C. , Chromosomal macrodomains and associated proteins: implications for DNA organization and replication in gram negative bacteria, Plos Genetics, vol. 7, no. 6, pp. e1002123.

Driessen, R.P., Dame, R.T. , Nucleoid-associated proteins in Crenarchaea, Biochemical Society Transactions, vol. 39, no. 1, pp. 116-21.

Dame, R.T., Tark-Dame, M., Schiessel, H. , A physical approach to segregation and folding of the Caulobacter crescentus genome, Molecular Microbiology., vol. 82, issue 6, pp. 1311-1315.

2010

Dame, R.T., Dorman, C.J., Eds., , Bacterial Chromatin, Text book: Springer, pp. 448.

Dame, R.T. , Ultrastructure and organization of bacterial chromosomes, Bacterial Chromatin: Springer, pp. 3-11.

2009

Wiggins, P.A., Dame, R.T., Noom, M.C., Wuite, G.J. , Protein-mediated molecular bridging: a key mechanism in biopolymer organization, Biophys J, vol. 97, no. 7, pp. 1997-2003.

Dame, R.T., van Mameren, J. , Optical tweezers: empowering biology with the force of light, Biophotonics, vol. 5, pp. 34-37.

2008

Luijsterburg, M.S., White, M.F., van Driel, R., Dame, R.T. , The major architects of chromatin: architectural proteins in bacteria, archaea and eukaryotes, Crit Rev Biochem Mol Biol, vol. 43, no. 6, pp. 393-418.

Dame, R.T. , Single-molecule micromanipulation studies of DNA and architectural proteins, Biochemical Society Transactions, vol. 36, pp. 732-737.

2007

Noom, M.C., Navarre, W.W., Oshima, T., Wuite, G.J.L., Dame, R.T. , H-NS promotes looped domain formation in the bacterial chromosome, Current Biology, vol. 17, no. 21, pp. R913-R914.

Dame, R.T., Noom, M.C., Wuite, G.J.L. , Bacterial chromatin organization by H-NS protein unraveled using a dual DNA assay, Biophysical Journal, pp. 26A-26A.

2006

Dame, R.T., Noom, M.C., Wuite, G.J.L. , Bacterial chromatin organization by H-NS protein unravelled using dual DNA manipulation, Nature, vol. 444, no. 7117, pp. 387-390.

Luijsterburg, M.S., Noom, M.C., Wuite, G.J.L., Dame, R.T. , The architectural role of nucleoid-associated proteins in the organization of bacterial chromatin: A molecular perspective, Journal of Structural Biology, vol. 156, no. 2, pp. 262-272.

Leliveld, S.R., Dame, R.T., Wuite, G.J.L., Stitz, L., Korth, C. , The expanded octarepeat domain selectively binds prions and disrupts homomeric prion protein interactions, Journal of Biological Chemistry, vol. 281, no. 6, pp. 3268-3275.

2005

Dame, R.T. , The role of nucleoid-associated proteins in the organization and compaction of bacterial chromatin, Molecular Microbiology, vol. 56, no. 4, pp. 858-870.

Dame, R.T., Luijsterburg, M.S., Krin, E., Bertin, P.N., Wagner, R., Wuite, G.J.L. , DNA bridging: a property shared among H-NS-like proteins, Journal of Bacteriology, vol. 187, no. 5, pp. 1845-1848.

Dame, R.T., Noom, M.C., Wuite, G.J.L. , A dual DNA molecule experiment reveals details of H-NS mediated bridging of DNA, Biophysical Journal, vol. 88, no. 1, pp. 381A-381A.

Dame, R.T., van Mameren, J., Luijsterburg, M.S., Mysiak, M.E., Janicijevic, A., Pazdzior, G., van der Vliet, P.C., Wyman, C., Wuite, G.J.L. , Analysis of scanning force microscopy images of protein-induced DNA bending using simulations, Nucleic Acids Research, vol. 33, no. 7, pp. -.

Dame, R.T., van Mameren, J., Luijsterburg, M.S., Mysiak, M.E., Janicijevic, A., Pazdzior, G., van der Vliet, P.C., Wyman, C., Wuite, G.J.L. , Analysis of scanning force microscopy images of protein-induced DNA bending using simulations (vol 33, pg 68, 2005), Nucleic Acids Research, vol. 33, no. 8, pp. 2767-2767.

2004

van Noort, J., Verbrugge, S., Goosen, N., Dekker, C., Dame, R.T. , Dual architectural roles of HU: Formation of flexible hinges and rigid filaments, Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 18, pp. 6969-6974.

Leliveld, S.R., Dame, R.T., Rohn, J.L., Noteborn, M.H.M., Abrahams, J.P. , Apoptin's functional N- and C-termini independently bind DNA, Febs Letters, vol. 557, no. 1-3, pp. 155-158.

Dame, R.T., Noom, M., Goosen, N., Wuite, G.J.L. , Compaction of DNA molecules by H-NS observed with optical tweezers, Biophysical Journal, vol. 86, no. 1, pp. 327A-327A.

Remus Th. currently supervises the following PhD students:

For more information on courses and internships have a look at the education section.

27/08/2010

Molecular Genetics